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KMID : 0380619900220070812
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Korean Journal of Food Science and Technology
1990 Volume.22 No. 7 p.812 ~ p.816
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Sorbitol Production by Zymomonas mobilis Immobilized in Calcium Alginate Gels and Glutaraldehyde
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Kim Hyun-Joong
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Abstract
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This study describes the sorbitol production with permeabilized cells of Zymomonas mobilis immobilized in Ca-alginate. Toluene treated cells lose activity of glucose-fructose oxidoreductase due to the leaking of enzyme from the cells. To prevent this leakage, the permeabilized cells were treated with 0.25% glutaraldehyde by stirring for 1 h at room temperature. A continuous process with glutaraldehyde treated cells was developed and no significant reduction in the degree of conversion occurred during 210 h operation. The productivities were estimated to be about 7.2∼7.5 g/l-h for sorbitol at dilution rate 0.18 h^(-1).
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